The Covid-19 pandemic has altered learning processes and other facets of classroom instruction. The development of students’ self-efficacy during the learning process is critical to describe in order to attain future learning goals and achievement. Self-efficacy measures the extent to which pupils can execute and complete specific tasks. This study was conducted on students from Tadris Biology Department at IAIN Ternate in North Maluku, Indonesia. The research sample consisted of 40 students, 25 of whom were female and 15 of whom were male. Prior to their usage, the instrument employed in the investigation had been declared valid and reliable by experts and practitioners. A self-efficacy questionnaire was used to collect data. The results indicated that on average, Biology students in the RQANI classroom had a self-efficacy score of 81.00 (quite confident). Female students scored higher on various facets of self-efficacy, including tenacity, the ability to adapt to difficult tasks, the ability to avoid situations and behaviors that are beyond their skills, as well as cognitive and affective abilities. Several components of self-efficacy, however, must be cultivated in students. The findings of this study are expected to provide insights to secondary and tertiary level teachers on the importance of improving students’ competence, abilities, and confidence, particularly amid this COVID-19 pandemic